In vitro shoot regeneration from leaves of Pyrus communis L. rootstock and cultivars

Abstract The influence of TDZ on adventitious shoot regeneration from leaves the pear cultivars Conference and Abate Fétel, rootstock Farold ® 87 was investigated. Our main aim to set up efficient in vitro protocols for all these genotypes by using expanding elongated cultures as starting plant material. best results terms percentage were achieved half-strength Murashige Skoog basal medium supplemented with 1 μM NAA, combined 13.5 (87.3%) (53.3%), 9 Fétel (68%). impact leaf organogenesis antibiotics timentin, cefotaxime, carbenicillin, alone or combination, usually used control Agrobacterium overgrowth, kanamycin, commonly selection putatively transformed plants, also evaluated be exploited future transformation trials. In general, use carbenicillin (475 mM), cefotaxime/carbenicillin (210/238 mM) cefotaxime (630 did not negatively affect efficiency Conference, 87, respectively. 4 kanamycin should suitable select shoots leaves, while a lower concentration different strategy applied Conference. We report new usable application biotechnologies genetic improvement rootstocks.